The general architecture of RNA-dependent RNA polymerase (RdRp) supports the specific polymerization of nucleotide triphosphates (NTPs) to a growing RNA chain through its structural and functional properties. RdRp is an enzyme that catalyzes the synthesis of RNA from an RNA template, playing a crucial role in the replication of RNA viruses.
The architecture of RdRp consists of a conserved structure resembling a right hand, with three domains: fingers, palm, and thumb. The fingers and thumb domains hold the RNA template, while the active site is located within the palm domain. This active site is responsible for the polymerization of NTPs.
RdRp recognizes and binds to specific sequences on the RNA template, ensuring the correct positioning of NTPs for polymerization. The enzyme undergoes conformational changes upon binding the RNA template, facilitating the formation of a catalytically active complex.
The specificity of RdRp for NTPs is primarily determined by the shape and electrostatic properties of the active site. The enzyme has a unique mechanism to discriminate between NTPs, allowing the incorporation of only the correct complementary NTPs into the growing RNA chain. The enzyme's fidelity is crucial for maintaining the integrity of the synthesized RNA.
In conclusion, the general architecture of RdRp enables the specific polymerization of NTPs to a growing RNA chain through its conserved structural domains, recognition of the RNA template, and active site properties. This ensures the accurate and efficient synthesis of RNA, critical for the replication of RNA viruses.
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do not add any more lactose and watch what transpires. note what happens and why this occurs. how could you re-activate the lacz gene?
The lacZ gene is responsible for the enzyme β-galactosidase which breaks down lactose. When no more lactose is added, the lacZ gene is not activated and the β-galactosidase enzyme does not break down lactose. To re-activate the lacZ gene, you would need to add lactose back in so that the β-galactosidase enzyme is activated and lactose is broken down.
Lactose is a disaccharide sugar composed of glucose and galactose, which is found in milk. Lactose can be hydrolyzed into glucose and galactose through the catalytic action of lactase enzymes. This reaction occurs in the small intestine, and the glucose and galactose are then absorbed and used as energy by the body.
When lactose is present, the lac operon is activated, and the genes involved in lactose metabolism are transcribed into messenger RNA. When lactose is absent, the lac operon is turned off, and these genes are not expressed.
To re-activate the lacZ gene, it is necessary to add lactose or a lactose analog such as IPTG to the culture medium. IPTG is an inducer of the lac operon that does not bind to the repressor protein, allowing the genes involved in lactose metabolism to be expressed even in the absence of lactose.
When lactose is present, the lac operon is activated, and the genes involved in lactose metabolism are transcribed into messenger RNA. When lactose is absent, the lac operon is turned off, and these genes are not expressed.
Therefore, if no more lactose is added to the culture medium, the lac operon will turn off, and the genes involved in lactose metabolism will not be expressed. This occurs because the repressor protein binds to the operator site of the operon, preventing RNA polymerase from transcribing the genes involved in lactose metabolism.
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vii. explain how a single neurotransmitter can elicit different responses at different postsynaptic cells.
The neurotransmitter released by a neuron can elicit different responses at different postsynaptic cells due to different receptor proteins on the postsynaptic cell.
The receptors on the postsynaptic cell are activated by different neurotransmitters, which then cause the cell to produce different responses. For example, some receptors on the postsynaptic cell might activate certain ion channels that cause an influx of calcium ions, while others might cause an influx of sodium ions or other substances.
These different responses can be triggered by different concentrations of the same neurotransmitter, depending on the receptor proteins present on the postsynaptic cell.
Furthermore, postsynaptic cells can also have different expression levels of receptors, which can lead to different responses to the same neurotransmitter. Therefore, a single neurotransmitter can produce different responses at different postsynaptic cells depending on the types of receptors present on the cell.
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how does the body decrease the blood vessel radius? how does the body decrease the blood vessel radius? vasodilation vasoconstriction cardiac muscle contraction valve closure
The body decreases the blood vessel radius by vasoconstriction.
A blood vessel is a tubular structure that transports blood throughout the body. Blood vessels are divided into three types: arteries, veins, and capillaries.
The heart pumps blood into the arteries, which then branch off into smaller arterioles that supply the capillaries.
Vasoconstriction is the process by which blood vessels constrict or narrow their diameter, increasing vascular resistance and decreasing blood flow.
It helps to control blood pressure and redirect blood flow to areas of the body that require it more urgently than other areas.
Blood vessels can constrict to various degrees, depending on the needs of the body. Vasoconstriction can be caused by a variety of factors, including hormones, drugs, and neurotransmitters.
For example, the hormone norepinephrine causes vasoconstriction by activating alpha-adrenergic receptors on the smooth muscle cells of blood vessels.
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onsuming foods with high glycemic index immediately after a bout of exercise will multiple choice question. ensure a slow rise in blood glucose. supply amino acids for muscle repair. rapidly restore glycogen stores. replenish sweat losses.
Consuming foods with high glycemic index immediately after a bout of exercise will c. rapidly restore glycogen stores.
Consuming foods with a high glycemic index such as boiled potatoes, white rice, white bread after exercise can quickly restore glycogen stores that are depleted during exercise. The glycemic index value depends on several factors, including dietary fiber content, amylose and amylopectin levels, fat and protein content, starch digestibility, and processing methods.
Glycogen is a storage form of glucose, where glucose is a simple sugar, a form of carbohydrates. Glycogen is an important fuel for athletes, because it is quickly broken down and used for energy.
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you decide to join a lab working on osteocalcin because it works on this hormone. you know from this and the next lecture many of its functions, target organs and receptors. can you present in one page what is/are the question(s) you would want to address and how would you tackle it/them?
My approach to investigating the functions of osteocalcin would involve a combination of molecular and cellular techniques, animal models, and clinical studies, with the ultimate goal of advancing our understanding of this hormone and its potential applications in medicine.
As a researcher joining a lab working on osteocalcin, there are several questions that I would want to address in order to further our understanding of this hormone and its role in the body.
Firstly, I would want to investigate the molecular mechanisms by which osteocalcin interacts with its target organs and receptors. This could involve studying the 3D structure of osteocalcin and its binding sites, as well as using techniques such as gene expression analysis and protein-protein interaction assays to better understand how osteocalcin influences cellular signaling pathways.
Secondly, I would be interested in exploring the functional roles of osteocalcin in various physiological processes, including bone formation, glucose metabolism, and energy homeostasis. This could involve using animal models and cell culture systems to study the effects of osteocalcin on different tissues and organs, as well as conducting clinical studies to investigate the potential therapeutic applications of osteocalcin in the treatment of metabolic disorders such as diabetes.
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the lipid-containing outer envelope surrounding the viral capsid of many animal viruses is derived from...
The outer envelope surrounding the viral capsid of many animal viruses is derived from the host cell's lipid bilayer.
This lipid bilayer is the same membrane that encloses the host cell. During the process of viral replication, the capsid and other components of the virus are assembled inside the host cell and a portion of the host cell's membrane is used to form the outer envelope of the virus.
This envelope, along with the capsid, helps to protect the genetic material of the virus, allowing it to be transported to another cell for infection. The envelope also contains viral proteins that aid in the attachment and fusion of the virus to the host cell.
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Select the correct statement about absorptiona. 30% ingested materials have been absorbed by the end of the large intestine.b. carbohydrates diffuse across the villus epithelium and are then passively transported into blood capillaries.c. if intact fats are transported across the villus epithelium, an immune response may be generated.d. Amino acid transport is linked to sodium transport.The answer could be one choice or multiple choices
The correct statement(s) about absorption is (D) Amino acid transport is linked to sodium transport.
Amino acids are transported across the villus epithelium in the small intestine, and this process is linked to sodium transport.
Amino acids, not proteins, are absorbed; proteins rely on prior digestion to amino acids. Most absorption of amino acids occurs in the jejunum; there is a lesser contribution from the ileum.
Amino acids are absorbed by a co-transport mechanism with sodium ions. Both sodium ion and amino acid combine with a cell surface protein receptor.
There are different receptors for the groups: neutral amino acids, basic amino acids, acidic amino acids
In addition, certain amino acids may have there own specific transporter e.g. proline. The receptor then conveys both molecules to the inside of the cell.
The energy for this transport is derived from the concentration gradient for sodium across the cell membrane. Na-K ATPase transporters actively and continuously pump sodium ions outwards to maintain the gradient.
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